For researchers, what steps are required to begin a project involving the use of pluripotent cells?
In order to work with human pluripotent stem cells, an investigator must receive the appropriate authorization from the Instituto de Salud Carlos III. First, you must obtain the authorization from your institutional ethics committee. Then, the research project must be evaluated by the corresponding Committee for Human Tissue and Cell Donation and Use of theInstituto de Salud Carlos III. Note this requirement of the Spanish law applies not only to human embryonic stem cells: Research with human iPS cells requires an explicit authorization from ISCIII.
Once the authorizations from the Technical Committee and the Advisory Committee for Human Tissue and Cell Donation and Use are received, the researcher is able to start working with pluripotent cells.
As a researcher, how do I obtain common pluripotent cell lines such as H1 or H9?
First of all, any researcher working with pluripotent stem cells must be authorized by the Instituto de Salud Carlos III.
Once the Committee for Human Tissue and Cell Donation and Use of ISCIII have approved a project, the researcher may then apply for the pluripotent cell lines at WiCell (www.wicell.org), the Wisconsin International Stem Cell Bank through this application form.
How do I, as a researcher, deposit iPSCs lines in the National Stem Cell Bank?
If a researcher wants to deposit iPSCs in the NSCB, the line must have been obtained through a project authorized previously by the Advisory Committee for Human Tissue and Cell Donation and Use of the ISCIII.
If the iPSCs meet the legal requirements, the applicant should fill an application with the detailed information confirming that the line fits with the quality criteria established by the NSCB and provide the required information.