Contact

Mario Soriano

microelectronica@cipf.es
TEL: +34 963 289 681
Ext. 2103 / 4309
FAX: +34 963 289 701

Services


 

  • Providing reinforcement and support in the clinical diagnosis of several pathologies:
  • Renal: diagnosis of primary and secondary glomerulopathies
  • Muscular: support in the diagnosis of congenital, mitochondrial, nemaline and metabolic myopathies by inclusion bodies (amyloid, Tay-Sachs, etc.)
  • Metabolic: study of hereditary pathologies such as mucopolysaccharidosis, neuronal ceroid lipofuscinosis, Anderson-Fabry disease by accumulation of myeloid bodies, etc.
  • Dermatological: differentiation of congenital epidermolysis bullosa types
  • Pathology of the peripheral nervous system: support in the diagnosis of Guillain–Barré syndrome (acute inflammatory demyelinating polyneuropathy) by alterations in the myelin
  • Hypophyseal: reinforcement in the diagnosis of pituitary adenomas
  • Identification of intranuclear and cytoplasmic viral particles
  • Observation of the structure and morphology of viruses, bacteria, exosomes, microvesicles, proteins and some chemical compounds.
  • Study of morphological effects (increase of vacuoles, autophagosomes, lipid droplets, etc.) of drugs in cell culture lines.
  • Monitoring of the proliferation, differentiation and integration of previously tagged (e.g. with GFP, RFP,...) stem cells that have been transplanted in target tissue.
  •  
  • Functional studies of some proteins: observation of morphological changes in experimental animals due to the absence or overexpression of a given protein.
  • Localization of protein expression at the cellular level (membrane, cytoplasm, nucleus, extracellular matrix, etc) by immunohistochemistry (with colloidal gold or DAB).

Offered services:

  • Sample preparation for Transmission Electron Microscopy
  • Dehydration with increasing ethanols and application staining with contrasting agents (osmium tetroxide and uranyl acetate)
  • Embedding in epoxy resins (araldite)
  • Ultramicrotomy: cutting of semithin sections for light microscopy and ultrathin sections for electron microscopy, with diamond knifes
  • Negative staining techniques and optimization with Glow Discharge
  • Immunohistochemistry for electron microscopy (immunogold), pre- or post-embedding
  • Observation, image capture and guidance during sample study at the TEM