Licenciado/a T3 - T3.04/2021
The aim of our laboratory is to understand retinal function, both in homeostasis and disease, by focusing our efforts on achieving the following objectives:
- Understanding the molecular mechanism underlying hereditary retinal dystrophies and correlating the phenotype of these patients with animal models with homologous genetic deficiencies.
- Developing therapeutic strategies based on the use of human pluripotent stem cells.
- Deciphering the molecular mechanisms of retinogenesis, especially photoreceptor specification, by studying the directed differentiation of pluripotent stem cells. Understanding the development of these highly specialized neurons will likely contribute to the identification of therapeutic targets which could be used to design efficient treatments for diseases such as retinal dystrophies.
The selected person will work on the research project: “Understanding the molecular mechanism of hereditary retinal dystrophies as a tool to find novel therapies”, led by Dra. Dunja Lukovic (code MS18/00033 financed by Carlos III Health Institute (ISCIII) performing the following tasks:
- Cell culture, maintenance and cryopreservation of iPS cells
- Maintenance of the cell culture laboratory (database, orders, preparation of solutions)
- Analysis and extraction of DNA, RNA, RT-PCR, Sequencing
- Analysis and extraction of proteins
- Immunohistochemistry, Immunocytochemistry
- Fluorescence and confocal microscopy (sample preparation, analysis)