Electron Microscopy
Core Facilities
The Electron Microscopy Service enables high-magnification and high-resolution imaging of any biological sample through different processing techniques:
The negative staining procedure allows the structure and morphology of viruses, bacteria, extracellular vesicles (exosomes, apoptotic bodies, and microvesicles), proteins, and polymeric nanoparticles to be observed.
Embedding animal or plant histological samples, cellular cultures, bacterial cultures, biological pellets, etc. in resin and taking semi-thin and ultra-thin sections allows the visualization of internal structures.
Descriptive studies of pathological samples help provide support to clinical diagnoses.
The preparation of vitrified fluid samples and their analysis by cryogenic electron microscopy (cryo-TEM) preserves their native biological structures for observation.
Immunogold analysis is used to detect antigens on the external side of extracelullar vesicles by using colloidal gold.
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- FEI Tecnai G2 Spirit Biotwin 120 kw transmission electron microscope.
- Morada digital camera (EMSIS).
- Gatan Cryo-TEM Holder (model: 626).
- Gatan dry pumping station (model: 655).
- Leica EM GP grid plunger.
- Fischione tomography holder (model: 2020).
- Leica EM FC6 cryochamber.
- Leica UC6 ultramicrotome.
- Leica ULTRACUT R ultramicrotome.
- Leica VT1000S vibratome.
- Leica EM AFS freeze substitution and low temperature embedding system.
- Leica EM TRIM specimen trimming device.
- Baltec MED 020 evaporation system.
- Negative staining procedures.
- Embedding in resin.
- Cutting semi-thin and ultrathin sections.
- Descriptive study of samples through transmission electron microscopy.
- Cryotransmission electron microscopy (cryo-TEM) of vitrified samples.
- Immunogold analysis of extracellular vesicles.
CONTACT US
Mario Soriano
microelectronica@cipf.es
963289680 ext. 4205, 4309, 2109
