ELECTRON MICROSCOPY CORE FACILITIES

Electron Microscopy

The Electron Microscopy Service enables high-magnification and high-resolution imaging of any biological sample through different processing techniques:

Negative staining procedure: Enables the observation of the structure and morphology of viruses, bacteria, extracellular vesicles (exosomes, apoptotic bodies, microvesicles), proteins and polymeric nanoparticles.

Embedding in resin and cutting semithin and ultrathin sections of histologic samples (animal and plant), cellular cultures, bacterial cultures, biological pellets, etc.

Descriptive study of pathological samples and support to clinical diagnosis.

Cryo-TEM: Preparation of vitrified fluid samples and study them preservating their native biological structures.

Immunogold for extracelullar vesicles: Detection of antigens in external side of EVs with coloidal gold

  • Equipment and Resources
  • Services
  • FEI Tecnai G2 Spirit Biotwin 120 kw transmission electron microscope.
  • Morada digital camera (EMSIS).
  • Gatan Cryo-TEM Holder (model: 626).
  • Gatan dry pumping station (model: 655).
  • Leica EM GP grid plunger.
  • Fischione tomography holder (model: 2020).
  • Leica EM FC6 cryochamber.
  • Leica UC6 ultramicrotome.
  • Leica ULTRACUT R ultramicrotome.
  • Leica VT1000S vibratome.
  • Leica EM AFS freeze substitution and low temperature embedding system.
  • Leica EM TRIM specimen trimming device.
  • Baltec MED 020 evaporation system.
  • Negative staining procedures.
  • Embedding in resin.
  • Cutting semi-thin and ultrathin sections.
  • Descriptive study of samples through transmission electron microscopy.
  • Cryotransmission electron microscopy (cryo-TEM) of vitrified samples.
  • Immunogold analysis of extracellular vesicles.

Find out more about our imaging services here:

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    Mario Soriano

    microelectronica@cipf.es

    963289680 ext. 4205, 4309, 2109